Free Energy Problem #2

2.  In a typical cell at 37^oC the concentration of ATP = 8 x 10^-3 M, ADP = 1 x 10^-3 M, and P_i = 8 x 10^-3 M. What is the actual free energy change (DG') for ATP hydrolysis under these conditions?

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• DG = - 31 kJ/mol + RT ln {[1x10^-3M][8x10^-3M]/[8x10^-3M]} = -49 kJ/mol M