Enzyme alchemy: the engineering of ivePRAI

Table of Contents:
structure comparison between PRAI and IGPS
"Alchemy" in a Nutshell
Background
Strategy for IGPS Structural Modification
IGPS to ivePRAI: Selection
ivePRAI: the "Golden" Product
Implications of Alchemy for Enzyme Engineering
References
Links of Interest
Based on Altamirano MM et al, "Directed evolution of new catalytic activity using the a/b-barrel scaffold."
Nature 403, 10 February 2000.
Nikki Jarrett, Biochemistry 462bH,11/20/00
Some structures require the CHIME
Dr. Don P. Bourque, instructor
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