Homework 4

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Answer Key in PDF format

Bioc471a/571a Homework 2 - Due at the start of class September 18

1. (2 pts) How might knowing the complete sequence of E. coli K-12 be useful in developing a rapid detection test for pathogenic strains of E. coli that have been found in contaminated food products?







2. (2 pts.) Are molecular markers such as RFLP, STS, or STRP, considered a genotype or a phenotype? Based on the pedigree genotyping data presented in figure 4.8 in the text, what would you conclude if the STRP pattern of one of the offspring were A/A? Explain.







For questions 3 and 4, use the data presented in figure 4.19 in the text (same as in Lecture 8; "Positional mapping of human disease gene").

3. (2 pts.) Is it possible to determine from the PCR results if the S4 or S5 polymorphism is responsible for the disease phenotype? Explain. If this couple were to have another child, what is the probability that the child would be afflicted with the disease?









4. (2 pts.) Based on the data in part c of the figure, what is the molecular basis for the disease? How would these data explain the observation that heterozygous individuals are clinically normal?








5. (2 pts.) Based on the data presented in the second lab practicum in Lecture 8 ("Mapping gene regulatory sequences"), which restriction enzyme segment contains the basal promoter activity required for transcription? Which restriction enzyme segment contains an "enhancer" function that stimulates promoter activity? Explain.