1. Protein has been purified based on a biochemical assay.
2. Isolate cDNA sequences encoding the gene ORF.
3. Generate antibodies using the cDNA.
4. Express the wild-type and mutant forms of the protein.
5. Determine the molecular mechanism of protein activity. |
1. Developmental gene is isolated from a model organism.
2. Use GenBank database to find orthologous genes in mammals.
3. Use ESTs to get cDNA and isolate genomic clones.
4. Construct transgenic mice with gene KO.
5. Determine developmental conseqeunces of gene defect in mice. |
1. cDNA isolated based on differential expression.
2. Isolate full-length cDNA and use with ESTs to build contig.
3. Use structure function analyses and cell transfection expts to study function.
4. Establish a genetic model in plants, flies or mice.
5. Determine the function of the encoded gene.
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1. Biochemical process is characterized by a cell-based assay.
2. Isolate cDNA sequences by expression cloning.
3. Isolate full-length cDNA and use with ESTs to build contig.
4. Use structure function analyses and cell transfection expts to study function.
5. Establish a genetic model in plants, flies or mice. |
1. A human genetic disease is mapped in a population.
2. Isolate disease locus using positional cloning methods.
3. Identify molecular basis for disease by correlating mutation to clinical phenotype.
4. Establish mouse model of human genetic disease.
5. Design a drug screen using this mouse model to develop new therapies. |
