Lec.

Date

Reading

Discussion Topic

1

Aug 21

pp. 3-10

Overview of Applied Molecular Genetics

2

Aug 23

pp. 12-22

DNA metabolizing enzymes; Biochemical methods
Homework 1

3

Aug 28

pp. 22-27

Nucleic Acids Lab Practicums

4

Aug 30

pp. 31-42

E. coli K12 bacterial host; Plasmid biology
Homework 2

5

Sep 4

pp. 42-55

DNA transformation; Bacteriophage vectors

6

Sep 6

pp. 59-78

Overview of DNA libraries; In vitro mutagenesis
Homework 3
 

7

Sep 11

pp. 83-96

Overview of Genomics; Genomic mapping

8

Sep 13

pp. 96-111

Genome Mapping Lab Practicums
Homework 4

9

Sep 18

pp. 115-127

Biochemistry of cDNA synthesis; Representative cDNA libraries

10

Sep 20

pp. 128-134

Functional cDNA libraries; Using cDNA as a reagent
Homework 5

11

Sep 25

pp. 134-138

cDNA Cloning Lab Practicums

-

Sep 27

-

471 & 571 Mid-term Exam (in class)

12

Oct 2

pp. 143-151

Biochemistry of the polymerase chain reaction (PCR)

13

Oct 4

pp. 151-158

Reverse Transcriptase-Mediated PCR
Homework 6

14

Oct 9

pp. 159-169

PCR Applications Lab Practicums

15

Oct 11

pp. 175-191

Gene expression in cultured cells
Instructions for Class Presentations

16

Oct 16

pp. 191-195

Yeast as a model; Protein expression in cultured cells

17

Oct 18

pp. 195-200

Gene Expression Lab Practicums
Homework 7

18

Oct 23

pp. 205-212

Molecular Genetics of Drosophila; Transgenic crop plants

19

Oct 25

pp. 214-229

Mouse transgenesis; Transgenic livestock
Homework 8

20

Oct 30

pp. 229-231

Transgenic Organisms Lab Practicums

21

Nov 1

pp. 237-244

Microarray Technology

22

Nov 6

handout

Computer Lab - Web Site Basics (meet in BSW 243)

23

Nov 8

pp. 244-250

Bioinformatics and Proteomics
Homework 9

24

Nov 13

pp. 250-253

DNA Forensics and Pharmacogenetics

25

Nov 15

pp. 253-262

Gene Therapy and Stem Cell Biology
Homework 10

-

Nov 20

-

Work on Group Projects

-

Nov 22

-

Thanksgiving Day Holiday

-

Nov 27

-

Class Presentations (Groups 2, 3 and 5)

-

Nov 29

-

Class Presentations (Groups 1 and 4)

-

Dec 4

-

471a & 571a Final Exam (in class)

-

Dec 13

-

Deadline for Written Lab Practicum
(Bioc 571a students only)
Turn them into Dr. Miesfeld in BioScience West room 518a