Lecture Notes Handout
Teng, p. 1-5, 89-94
Marley et al. article
Marley et al. Reading Guide
Wuthrich Nobel lecture (p. 235-237)

Cryogenic Probe Info

Thursday
Jan 24, 2008

NMR Basics II:
All at once: Pulse NMR, the Fourier transform,
and the vector formalism

Lecture Notes Handout
Teng, p. 6-17
Pulse NMR handout, p. 9-20 (21-26 are optional for now)

None

NMR Basics III:
A simple, single pulse 1D experiment

 

Lecture Notes Handout
Teng, p. 17-22
(Teng reading is really a primer for Tuesday on chemical shift)

None

Tuesday
Jan 29, 2008

NMR Fingerprints of Proteins:
Interpreting simple spectra; chemical shifts

Lecture Slides (Powerpoint)
Shuker article, reading guide
Assessing 1-dimensional Spectrum (handout)
Wuthrich Nobel lecture (p. 238-242)
Hill article (optional)

None

NMR Basics IV:
Multidimensional NMR spectra

Lecture Notes Handout
Teng, p.136-137

None

Tours of Varian 600 MHz NMR (optional)
10 am and 4 pm, Old Chem 121D

Teng, Chapter 2; Experimental Aspects handout

Sign up by
emailing Dr. Cordes

Computer lab 1:
Comparing HSQC spectra to gauge effect of a mutation

No new reading

None

Illustration of INEPT and HSQC pulse sequences

Resonance assignment, Part I:
1H scalar couplings, spin systems,
and 2D COSY/TOCSY spectra

lecture slides (PPT file)
Spin systems and INEPT/HSQC handouts
Teng, p. 23-26 (scalar couplings)
Teng, p. 42-47 (coherence transfer--vector and product operator)

Prob Set 1 due Feb 12

(handed out in class)

NMR Basics V:
The spin-dipolar coupling
and the nuclear Overhauser effect

Lecture notes handout
Teng, p. 27-top 28, p.32-35
(Dipolar coupling and NOE)

None

Resonance assignment, Part II:
Approaches to sequence-specific assignments

None

Tuesday
Feb 12, 2008

Computer lab 2:
Assigning and connecting spin systems of a tryptophan residue

Lab handout (distributed day of class)
NOE distances handout

Meet in BSW 243