Copyright 1984-1998 FileMaker, Inc. HBAM2016AUG95HPro 3.0w5_md1@uq @`` @`argeprettyturbidthereisalargeless-turbidbutnotcle@largeturbidveryslightclearishdotincenter@or@-sl@ ggct@"tcag@: gtct@B0g#EX STRAIN NAME'@4 B&A+ AJ @XJ )@MOcMBA@S(3@ V6a7m;A@CI@85MDUEG@CYOAR@PZQN]TAW@UPVS KZU[_\i]@Y  A%@9M @ U @C@1E/ @F#D$$@'G*@>-@/H23@?9H<KG@‚s?mdU G0H@IPJ`KpLMNOmdcdcmd15&md16'md17(md18)md19*md201md212md223md234md245md256md267md278md289md29:md30Amd31 B md32!C!md33"D"md34#E#md35$F$md36%G%md37&H&md38'I'md39(J wwbTwbT"ctYAMD1B1CMDGyesHlarge, turbid, ~like WTJTCCTKTCCTMTNCOCPTQTRCSCTTU0V1W7]1^2_5`2"A2CAACACGCACGGTGTTATCCTTTTATCCCTTGCGGTGATATCCTTAACGT2@Af      (  ,  fcturbid, about like WTdWTe4hyesiturbidjlargek4 ++++AMD3B3CMDGyesHlarge, turbid, ~ like WTJGACTKTCTCMGNAOCPTQTRCSTTCU2V1W5]3^1_4`2b2CAACACGCACGGTGTTAGACTTTTATCCCTTGCGGTGATATCTCTAACGTc#turbid, larger clear center than WTd=WTe4.5hyesGCATsX>         A  $,.YesNo/,  , : AM PMB   C $,.YesNo/,  , : AM PMD   E $,.YesNo/,  , : AM PMF   G $,.YesNo/,  , : AM PMH   I $,.YesNo/,  , : AM PMA(B strain nameNplaque phenotypePlysogenization?ZpRM -35\-10^totaldiffs from WT_WT=GATA`GATTbdiffs fromconsd AT bp in -10 @B!A B!&A BG      A pRM sequencesB ( @(A $,.YesNo/,  , : AM PMwB    E $,.YesNo/,  , : AM PMwF    M $,.YesNo/,  , : AM PMN   $,.YesNo/,  , : AM PMN   N   $,.YesNo/,  , : AM PMN   N   $,.YesNo/,  , : AM PMN   N   $,.YesNo/,  , : AM PMN   N   M O $,.YesNo/,  , : AM PM33P   Y $,.YesNo/,  , : AM PMwZ  [ $,.YesNo/,  , : AM PMw\  ] $,.YesNo/,  , : AM PMw^  _  `  a $,.YesNo/,  , : AM PMwb  l1PTQCRASATAU0V1W7]1^3_4`4b2CAACACGCACGGTGTTATCCCTTTATCCCTTGCGGTGATATAAATAACGThunstableiturbidjlargek4 ++++ 2AMD49B49CMDGyesHcmedium, turbid, possibly more uniform than WT. Another test, very slight less-turbid dot in centerI0Plaques more uniform in turbidity across plaque.JGTCAKTAAACMGNTOCPTQARASATAU2V1W5]3^3_2`4b3CAACACGCACGGTGTTAGCTATTTATCCCTTGCGGTGATATAAACTAACGTd~WTe4hyesiturbidjmediumk4 ++++3AMD50B50CMDGunstableH large, turbidJACAAKTCTTMANCOAPTQARCSTTTU1V2W5]1^2_5`3"A2CAACACGCACGGTGTTAACAATTTATCCCTTGCGGTGATATCTTTAACGT2@A>      >hunstableiturbidjlargek4 ++++ 4AMD51B51CMDGyesH large, turbidJTACCKTCATMTNAOCPTQCRCSATTU1V1W6]2^3_3`3b2CAACACGCACGGTGTTATACCTTTATCCCTTGCGGTGATATCATTAACGTdlarge, pretty turbidJCATGKGAATMCNAOTPGQGRASATTU2V3W3]1^3_4`3b2CAACACGCACGGTGTTACATGTTTATCCCTTGCGGTGATAGAATTAACGTd ~WT or >WTe4hyesiturbidj med to largek3 +++AMD29B29CMDGyesHlarge, pretty turbidJGATAKGATTMGNAOTPGQARASTTTU4V4W0]3^2_3`3b2CAACACGCACGGTGTTAGATATTTATCCCTTGCGGTGATAGATTTAACGTd ~WT or >WTe4hyesi pretty tujlargek3 +++AMD30B30CMDGyesHlarge, turbid, ~WTJCCCAKCAGTMCNCOCPCQARASGTTU1V2W5]2^2_4`2"A2CAACACGCACGGTGTTACCCATTTATCCCTTGCGGTGATACAGTTAACGT2@A>  *  0  >cslightly different from WTd>WTe5hyesiturbidjlargek4 ++++ AMD31B31CMDGno 3/24 no, but 1 colonyHjlarge, turbid, relatively uniform across plaque, more clear in center but no clearish border (like WT has)I/Plaques are uniform in turbidity across plaque.JCAACKGGACMCNA *iturbidjlargek4 ++++AMD5B5CMDGyesHlarge, turbid, ~ like WTJATACKGACTMANTOAPGQCRASCTTU0V3W5]0^2_6`2b2CAACACGCACGGTGTTAATACTTTATCCCTTGCGGTGATAGACTTAACGTc#turbid, larger clear center than WTd <<wtIOIn lysogeny test, streak is thin and watery-looking, colonies are small to tinyJTCTCKGAGTMTNCOTPGQCRASGTTU1V3W4]0^2_6`2"A2CAACACGCACGGTGTTATCTCTTTATCCCTTGCGGTGATAGAGTTAACGT2@A>  *  0  >hunstableiturbidjlargek5 +++++ AMD9B9CMDGyesZ OAPGQCRGSATCU1V1W6]1^1_6`1b2CAACACGCACGGTGTTACAACTTTATCCCTTGCGGTGATAGGACTAACGThnoiturbidjlargek4 ++++ !AMD32B32CMDGyesHlarge, turbid, ~WTJTAGCKACATMTNAOGPAQCRCSATTU1V1W6]1^2_5`3b2CAACACGCACGGTGTTATAGCTTTATCCCTTGCGGTGATAACATTAACGTc#turbid, larger clear center than WTd<WTe4hyesi pretty tujlarge?k3 +++#AMD34B34CMDGyes, rel few but healthyHv faintly turbid in center; some are larger and clear (may be clears). After two days, more turbid in center. Looks like a cII mutant might look.ISTwo oR3 mutations -- from oR2 side: 1st posn T->C 9th posn G->T No mutation in cIIJGACCKGTGTMGNAOCPGQCRTSG!xlargeprettyturbidthereisalargeless-turbidbutnotcleKVlargeprettyturbidwtkVlargeturbidXVsVtVuVvV|VlargeturbidabitwtpossiblyafewhetsPVlargeturbidlikewtBVDVEVFVlargeturbidlikewtnothetNVlargeturbidlikewtorpossslwtOVlargeturbidpossslwtCVlargeturbidrelativelyuniformacrossplaquemoreclearin`VjVjVturbidvturbidVshdotincenterJVlargeturbidwtQVTVZV_VaVfVnVqVlargeveryturbidposswt[VlargeveryturbidpossiblywtIVlargercWeWless-turbidKWlikeBWDWEWFWGWNWOW`WcWeWjWlookGWcWeWlookscWeWmaycWeWmedium\W]WrWmediumlargeprettyturbid]VmediumturbidpossiblymoreuniformthanwtrVmedium-sizedmWmedium-sizedfairlyturbidmVmightcWeWmore`WcWeWjWrWmostGWmutantcWeWno`WjWnotKWNWongWorOWpWplaqueLW`WjWpossCWOW[WpossiblyIWPWrWprettyKWMW\W]W^WbWhWkWoWpWprettyturbidwtbVrelativelyLW`WjWB[0')*-.3=>@ ,/5;?O` )-/23:;HKNp !"$%&'(+489GM0167IJL """y!,.56""aacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacgaacacgcacggtgtagacg,,@MSUDHP LaserJet IIId A4d</ *OA  CTCCKCCATMCNTOCPCQCRCSATTU0V1W7]1^2_5`2"A2CAACACGCACGGTGTTACTCCTTTATCCCTTGCGGTGATACCATTAACGT2@Af      (  ,  fchtwo types when coplated; probably 53 has larger clear center than WT, but cant be sure which is lighterd>WTe5hyesiturbidjlargek++++bid JCTCCKCCATMCNTOCPCQCRCSATTU0V1W7]1^2_5`2"A2CAACACGCACGGTGTTACTCCTTTATCCCTTGCGGTGATACCATTAACGT2@Af      (  ,  fchtwo types when coplated; probably 53 has larger clear center than WT, but cant be sure which is lighterd>WTe5hyesiturbidjlargek++++hen coplated; probably 53 has larger clear center than WT, but cant be sure which is lighterd>WTe5hyesiturbidjlargek+++++++ or ++++>fN-1yslCWLWOWslightJWrWsmallMWWW\Wsmallmediumprettyturbid\VsomecWeWtestrWthanrWtheLWthereKWturbidBWCWDWEWFWHWIWJWKWLWMWNWOWPWQWRWSWTWUWVWWWXWYWZW[W\W]W^W_W`WaWbWcWdWeWfWgWhWiWjWkWlWmWnWoWpWqWrWsWtWuWvWwWxWyWzW{W|W}W~WWturbidon3/24prettyturbidon2/6/04gVtwocWeWuniformLW`WjWrWvcWeWvfaintlyturbidincentersomearelargerandclearmaybecVeVveryIWJW[WiWrWyW{WwideWwtBWCWDWEWFWIWNWOWPWQWTWZW[W_W`WaWbWfWjWkWnWqWrWzoneKW 1stcW4MW4/6/01QWnW6OW9thcWthcWWhcWWthcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWcWWWWWWthcWWWthcWWthcWWWWWWWWWWWKWbeen`WcOWcWcheckNWcoloniesHWIWKWcolony`Wcould`WdoneHWKW`WeffectHWKWfairlyRWfrom`WcWhasOWhasanor2mutationor1sideposition6ctOVhave`WhetNWi`WbhealthyWW\WcWfWgWxWno $)*?notGWHWIWJWKWLWMWNWOWnottestedGVHVIVJVKVLVMVNVOVrelWW\WcWfWgWxWtestedGWHWIWJWKWLWMWNWOWunstable 0139yes WWbitPWborder`WjWbutKW`WjWcenterHWJWMW`WcWeWjWcentralKWLWciicWeWclearHWKW`WcWeWjWclearishJWLWMW`WjWclearscWeWdayscWeWdotHWJWLWMWfaintlyHWLWcWeWgWfaintlyturbidlargecleardotincenterHVfairlyGWRWlWmWpWfairlyturbidpVfairlyturbidmostlooklikehetsGVfewPWhas`WjWhetNWhetsGWPWinHWJWMW`WcWeWjWisKWlargeBWCWDWEWFWHWIWJWKWLWMWNWOWPWQWRWTWZW[W]W^W_W`WaWfWgWhWjWkWlWnWoWlargefaintlyturbidgVlargefaintlyturbidrelativelyuniformacrosstheplaqueslLVlargefairlyturbidRVlVlargeprettyturbid^VhVoVlargeprettyturbidsmallclearishdotincenterMV9nb2CAACACGCACGGTGTTAGCACTTTATCCCTTGCGGTGATAAACTTAACGTc#turbid, larger clear center than WTd>WTe5hyesiturbidjlargek4 ++++AMD20B20CMDGyesHlarge, pretty turbidJGCTTKGAAGMGNCOTPGQTRASATGU2V2W4]1^2_5`2b2CAACACGCACGGTGTTAGCTTTTTATCCCTTGCGGTGATAGAAGTAACGTd <<  *  0  >d<  *  0  >d<yesrelfewbuthealthyWV\VcVfVgVxV2/6/04dWgW2/6/04largefairlyturbidclearishdotincenterdV3/24gWaKWPWcWeWacrossLW`WjWaftercWeWandWWcWeWanotherrWarecWeWaroundiWbecWeWbitPWborder`WiWjWWbutKW`WjWcenterHWJWMW`WcWdWeWjWrWxW~WWcentralKWLWciicWeWclearHWKW`WcWeWjWWGWHWIWJWKWLWMWNWOWclearplaquelittleornogrowthonjl5434GVHVIVJVKVLVMVNVOVPVPVGVHVIVJVKVLVMVNVOVPVVVVPVVJVKVLVMVNVOVPVVVVJVKVLVMVNVOVPVVVGVHVIVJVKVLVMVNVOVPVVVVWhas`WjWhetNWhetsGWPWinHWJWMW`WcWeWjWrWxW~WWisKWjl5434GWlargeBWCWDWEWFWHWIWJWKWLWMWNWOWPWQWRWSWTWUWVWWWXWYWZW[W]W^W_W`WaWfWgWhWiWjWkWlWnWoWpWqWsWtWuWvWwWxWzW|W}W~WWdotincenterMV-"A2CAACACGCACGGTGTTATCCATTTATCCCTTGCGGTGATAAAGTTAACGT2@A>  *  0  >d1.5  *  0  >hunstablei pretty tujlargek3 +++1AMD48B48CMDGunstableHlarge, turbid ~WTJTCCCKTAAAMTNCOC )b2CAACACGCACGGTGTTACCCATTTATCCCTTGCGGTGATAGGTATAACGThnoifaint tujlargek1 +*AMD41B41CMDGnoHjlarge, turbid, relatively uniform across plaque, more clear in center but no clearish border (like WT has)I+Plaques are uniformly turbid across plaque.JCATGKGAGAMCNAOTPGQGRASGTAU2V2W4]1^1_6`2b2CAACACGCACGGTGTTACATGTTTATCCCTTGCGGTGATAGAGATAACGThnoiturbidjlargek4 ++++ +AMD42B42CMDGyesHlarge, pretty turbid, WTe5hyes, fewiturbidjlargek4 ++++'AMD38B38CMDGyes, rel few but healthyHDlarge, faintly turbid turbid on 3/24 pretty turbid (+++) on 2/6/04JACCTKTAGTMANCOCPTQTRASGTTU0V2W6]1^3_4`3"A2CAACACGCACGGTGTTAACCTTTTATCCCTTGCGGTGATATAGTTAACGT2@Af      *  0  fd<wtIOIn lysogeny test, streak is thin and watery-looking, colonies are small to tinyU0V0W8[0[0[0[0.R)A4dCustom page 1XCCCustom page 2XCCCustom page 3XCCfA4dPQ streakIWtOWcWtempKWtemperatureKWtestHWIWKWthinIWtinyHWIWtoIWturbidjWturbidity`WrWtwocWtwoor3mutationsfromor2side1stposntc9thposngtcVuniformLW`WrWuniformlyjWvHWveryKWwatery-lookingIWxhoieWzoneHWJWKWMWWMWWid  A#F#:E ^#KC  A$A )95  0):9h BDT)q9c:>_ (9!0"(:# $4+ B G A pRM min10B G A min35pos3B A>( 11)A ??>A min35pos4B A>( 21)A @?>A min35pos5B A>( 31)A @?>A min10pos3B A>( 11)A ??>A min35pos6B A>( 41)A @?>A min10pos4B A>( 21)A @?>A min10pos5B A>( 31)A @?>A min10pos6B A>( 41)A @?>A min35likeWTB 4 STRAIN NAME TOTALDIFFS TURBIDITY+TYPEVALUE FOR MITC SPOT TEST% "#$()*+%    DA strain nameB A & F()AFGAnumberB GL12AtypeB A"MDC"AMDCGAmutagenesis methodB A"doped PCR primers"Adoped PCR primersGA isolated byB A"JWL"AJWLGA date isolatedB GAlysogenization?B GAplaque phenotypeB G AcommentsB G A pRM min353 S $,.YesNo/,  , : AM PMwT   U $,.YesNo/,  , : AM PMwV   W $,.YesNo/,  , : AM PMwY $,.YesNo/,  , : AM PMw[ $,.YesNo/,  , : AM PMw\    A bP̙X. p ,H'I p&' pl&,<I p.i;@Q^J  Bp@MO,A +0, R> H1large, turbid, very slight clearish dot in centerI#Plaques have clearish central zone.JTCAGKGAGTMTNCOAPGQGRASGTTU0V3W5]0^2_6`2"A2CAACACGCACGGTGTTATCAGTTTATCCCTTGCGGTGATAGAGTTAACGT2@A>  *  0  >d  *  0  >hnoi pretty tujlargek3 +++ AMD11B11CMDGunstableHUlarge, faintly turbid, relatively uniform across the plaque, sl. clearish central dotAMDC9&J A@G( "G")+G("A")+G("T")+G("A")@A GGAGTGAGA min10likeWTB AAG( "G")+G("A") +G("T")+G("T")AAGGAGTGTGA totaldiffsB A 8-- A@ A min35likeconsB A@G( "G")+G("A")+G("C")+G("A")@A GGAGCGAGA min10likeconsB AAG( "T")+G("A") +G("A")+G("T")AATGAGAGTGA diffsfromconsB A 8-- A@  A ATinmin10B AG("A")+G("T")+ G("A")+G("T")+ G("A")+G("T")+ G("A")+G("T")!+clearishJWLWMW`WdWjWclearscWeWdayscWeWdotHWJWLWMWdWrWxW~WWfaintlyHWLWWWcWeWgWiWfaintlyturbidlargecleardotincenterHVfairlyRWdWiWmWwWxW}W~WWfairly-turbid~WfewPWgrowthGWHWIWJWKWLWMWNWOWhas`WjWhetNWhetsPWinHWJWMW`WcWdWeWjWrWxW~WWisKWjl5434GWHWIWJWKWLWMWNWOWlargeBWCWDWEWFWHWIWJWKWLWMWNWOWPWQWRWSWTWUWVWWWXWYWZW[W]W^W_W`WaWdWfWgWhWiWjWkWlWnWoWpWqWsWtWuWvWwWxWzW|W}W~WWWWWWWxWzW|W}W~WWWWWWWzorOWGWHWIWJWKWLWMWNWOWotherWWplaqueLW`WjWGWHWIWJWKWLWMWNWOWplaquesWWplaquesmoreturbidandsmallonothersideofplateWVplateWWpossCWOW[WpossiblyIWPWrWprettyKWMWSWUWVWYW\W]W^WbWgWhWkWlWoWpWzWprettyturbidlargelVpVprettyturbidwtbVpretty-turbidWrelativelyLW`WjWsideWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWf#B|Custom page 1XCCCustom page 2XCCCustom page 3XCCfA4dsOB9CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JCATAKGGGGMCNAOTPGQARGSGTGU3V1W4]2^0_6`0b2CAACACGCACGGTGTTACATATTTATCCCTTGCGGTGATAGGGGTAACGThNTicleark0 - PAMDC10@A  B10CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JCCCGKGATGMCNCOCPGQGRASTTGU0V3W5]1^1_6`2b2CAACACGCACGGTGTTACCCGTTTATCCCTTGCGGTGATAGATGTAACGThNTicleark0 - 5 .9139311 .9003245 .8869205 .8737159 .860708 40 .9801987 .9607894 .9417645 .9231163 .9048374 .8869205 .8693582 .8521438 .8352702 .8187307 50 .97531 .9512295 .9277435 .904837UB0CMDCI(spacer for columns)>hNTicleark-NAMDC8@A  B8CMDC >hNTicleark-NAMDC8@A  B8CMDCMDCMDCrH ABCDEFGHI J K L M NOPQRSTUVWXYZ[\]^_` a!b"c#d$e%f&g'h(i)j*k+ AB C    !"#$%&'()*+,-./0123456789:;<=>?GHIJKLMNOU"  "   "            "O# aKWMWacross`WjWrWanOWandIWareIWLW`WjWatMWcOWcWcentralHWJWKWMWcieWMWciicWeWxWclearHWGWHWIWJWKWLWMWNWOWclearplaquelittleornogrowthonjl5434GVHVIVJVKVLVNVOVclearplaquelittleornogrowthonjl5434hasamutationinMVclearishJWMWcoloniesHWIWKWcolumnsUWdayHWKWe10veWeffectKWforUWfromcWfsMWgcWgrowthGWHWIWJWKWLWMWNWOWhasOWMWhasanor2mutationor1sideposition6ctOVhaveHWJWKWMWMWMWMW?L?"A2CAACACGCACGGTGTTAACTCTTTATCCCTTGCGGTGATAGAGTTAACGT2@Af      *  0  fhnoi fairly tujlargek2 ++ GAMDC1@A  B1CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JAAATKTTTGMANAOAPTQTRTSTTGU1V1W6]1^1_6`3b2CAACACGCACGGTGTTAAAATTTTATCCCTTGCGGTGATATTTGTAACGThNTicleark0 -HAMDC2@A  B2CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JACTAKGGGGMANCOTPGQARGSGTGU2V1W5]1^0_7`0"A2CAACACGCACGGTGTTAACTATTTATCCCTTGCGGTGATAGGGGTAACGT2@A>      >hNTicleark0 -IAMDC3@A  B3CMDCG not testedGTCGTC( DA,I'`L' ` '= `8, $,.YesNo/,  , : AM PMw $,.YesNo/,  , : AM PMw $,.YesNo/,  , : AM PMw       '&          "#$%"(#)$*%+DA strain nameB A & F()AFGAnumberB GL16AtypeB A"MDC"AMDCGAlysogenization?B GAplaque phenotypeB G AcommentsB G A pRM min35 min35 min35 min35plaque phenotypeB G AcommentsB G A pRM min35 min35entsB G A pRM min35 min35 AcommentsB G A pRM min35 min358DA pRM sequencesB ( @,A $,.YesNo/,  , : AM PMwB  C  $,.YesNo/,  , : AM PMwD  E  $,.YesNo/,  , : AM PMwF  G  $,.YesNo/,  , : AM PMwH  I  $,.YesNo/,  , : AM PMwJ  7 dV   W $,.YesNo/,  , : AM PMwY $,.YesNo/,  , : AM PMw[ $,.YesNo/,  , : AM PMw] $,.YesNo/,  , : AM PMw_ $,.YesNo/,  , : AM PMw6; a $,.YesNo/,  , : AM PMwc $,.YesNo/,  , : AM PMwe $,.YesNo/,  , : AM PMwg  h  i $,.YesNo/,  , : AM PMwj  k $,.YesNo/,  , : AM PMwl  I51B strain nameT pRM min35V pRM min10g-35h-10jlike WT: -35 l-10.A total diffs @AH J?4Hplike cons: -35r-104Adiffs from consensus@AH ?H @BABA" "B6                   B strain nameT pRM min35V pRM min10g-35h-10 @BA  BA >NCOGPGQCRTSATTU0V2W6]0^2_6`3"A2CAACACGCACGGTGTTAACGCTTTATCCCTTGCGGTGATAGTATTAACGT2@A>      >d1.5aatt acat!acct:acgaIacggJcaac9cagtccat6cgat7ctct+gaac$gaaggaatgactgaga*gagt 5;?gatgNgattgcatgcttKggac ggctggggHOggta)gtat..t..-/1457=a*1IMc9gGHJLNOPQt #+,-./0345678:;<=K  !"$%&'()2>?IJLM +0146789:GHOQ0#*-.3=@ ,/5;?LAAz(HHR[ (hh t2 HK'd!5So zA4'd7@ m $,.YesNo/,  , : AM PMwn , o $,.YesNo/,  , : AM PMwp  q $,.YesNo/,  , : AM PMwr  s $,.YesNo/,  , : AM PMwt * @&@&LLKTGAGMANCOGPTQCRGSATGU0V0W8]0^2_6`2"A2CAACACGCACGGTGTTAACGCTTTATCCCTTGCGGTGATATGAGTAACGT2@A>      >hNTicleark0 - MAMDC7@A  B7CMDCG not testedH+clear plaque; little or no growth on JL5434IVclear plaque; little or no growth on JL5434. Has a mutation in cI -- fs at position 4JACTAKTGCAMANCOTPTQARGSCTAU2V0W6]1^1_6`2"A2CAACACGCACGGTGTTAACTATTTATCCCTTGCGGTGATATGCATAACGT2@A>      >hNTicleark0 -NAMDC8@A  B8CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JCCCGKGATGMCNCOCPGQGRASTTGU0V3W5]1^1_6`2b2CAACACGCACGGTGTTACCCGTTTATCCCTTGCGGTGATAGATGTAACGThNTicleark0 - OAMDC9@ANTOAPGQCQC^i Aphenotypes--landscapeBz @$A  $,.YesNo/,  , : AM PMB   M $,.YesNo/,  , : AM PMN   O $,.YesNo/,  , : AM PM33P   Y $,.YesNo/,  , : AM PMwZ  [ $,.YesNo/,  , : AM PMw\  *9 IPlaques are uniform.JCCCAKGTTTMCNCOCPGQARTSTTTU1V3W4]2^1_5`3b2CAACACGCACGGTGTTACCCATTTATCCCTTGCGGTGATAGTTTTAACGThunstableifaint tujlargek1 + AMD12B12CMDGunstableH2large, pretty turbid, small clearish dot in centerI)Plaques have small clearish central zone.JCATCKAATTMCNAOTPAQCRASTTTU2V3W3]1^2_5`4b2CAACACGCACGGTGTTACATCTTTATCCCTTGCGGTGATAAATTTAACGThunstablei pretty tujlargek3 +++ AMD13B13CMDGunstableH large, turbid, ~like WT, not hetJTGCCKTTTTMTNGOCPTQCRTSTTTU0V2W6]1^2_5`4b2CAACACGCACGGTGTTATGCCTTTATCCCTTGCGGTGATATTTTTAACGThunstableiturbidjlargek4 ++++ AMD14B14CMDGyesH&large, turbid, ~like WT or poss sl TJGCATKGACTMGNCOAPGQTRASCTTU1V3W4]1^2_5`2b2CAACACGCACGGTGTTAGCATTTTATCCCTTGCGGTGATAGACTTAACGTd>WTe5hyesiturbidjlargek4 ++++AMD15B15CMDGyesH-large, turbid, a bit   *  0  >d<AMD61B61CMDGyesH8large, fairly turbid (large fairly-turbid dot in center)JGAACKAAGTMGNAOAPAQCRASGTTU2V2W4]2^2_4`3"A2CAACACGCACGGTGTTAGAACTTTATCCCTTGCGGTGATAAAGTTAACGT2@A>  *  0  >d_ (9!0");# #$ Bd*:N ):!0"R: gtctgtgt#gttt ,/taaa&1taaac2tagatagt"'tatctattt !-/1457=a  !#*458:<>GIJOc "$%'),-139=?HLMNg &./0;t(+267Ka !$&.124679Lc(+:Mg "#%'*-058;=>?HIJOt ),/3?KIJLM  !&(+01246789:GHO0"#$%%%%%%%%%%%o zA4'd' GNEB strain nameNlysogenization?Pplaque phenotypeTpRM -35V-10 @BA   BA  B'  A for phylogenyB  @,A  $,.YesNo/,  , : AM PMB   P   P   $,.YesNo/,  , : AM PM33P   $,.YesNo/,  , : AM PM33P   5E <M $,.YesNo/,  , : AM PMwN  O  $,.YesNo/,  , : AM PMwP  S $,.YesNo/,  , : AM PMwT  $,.YesNo/,  , : AM PMwT  T  $,.YesNo/,  , : AM PMwT  T  DG U $,.YesNo/,  , : AM PMwV  W  $,.YesNo/,  , : AM PMwX  Y  $,.YesNo/,  , : AM PMwZ  [  $,.YesNo/,  , : AM PMw\  ]  $,.YesNo/,  , : AM PMw^  jk= B strain namehphenotype on MitCjMitC spot testlvalue for MitC spot test @B!A  B!AB5#$%otype on MitCjMitC spot testlvalue for MitC spot test @B!A B!A   B5        EC A"^`Xf" `f"`,$ ` #8 `0B`C `> `C `>0        "%&345 9; "'(567 ;= `>`>`>"`>"@8 C @1\-@AX.#j4/j0#412#434x@"}2B@"\2s@"2!@"2#@"2%@*?'(!1)@*!15+@*,+  A?AGTGAGTGAGTGAGTG?"AsequenceB A<"CAACACGCACGGTGTTA"& &"TTTATCCCTTGCGGTGATA"& &"TAACGT"<A:CAACACGCACGGTGTTA TTTATCCCTTGCGGTGATA TAACGT:G#Aphenotype on MitCB G$AMitC spot testB G%Avalue for MitC spot testB G(Alysogenization2B G)A clearvsturbidB G*A plaque sizeB G+A turbidityB G+A turbidityB G+A turbidityB G+A turbidityB Gaj B  @,A $,.YesNo/,  , : AM PMB   g $,.YesNo/,  , : AM PMwg $,.YesNo/,  , : AM PMwg $,.YesNo/,  , : AM PMwg $,.YesNo/,  , : AM PMw'acct$2<IH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JCACGKACGAMCNAOCPAQGRCSGTAU1V0W7]2^0_6`2b2CAACACGCACGGTGTTACACGTTTATCCCTTGCGGTGATAACGATAACGThNTicleark0 - JAMDC4@A  B4CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JCACCKACGGMCNAOCPAQCRCSGTGU1V0W7]2^0_6`1b2CAACACGCACGGTGTTACACCTTTATCCCTTGCGGTGATAACGGTAACGThNTicleark0 - KAMDC5@A  B5CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JATACKGCTTMANTOAPGQCRCSTTTU0V3W5]0^1_7`2b2CAACACGCACGGTGTTAATACTTTATCCCTTGCGGTGATAGCTTTAACGThNTicleark0 - LAMDC6@A  B6CMDCG not testedH+clear plaque; little or no growth on JL5434I+clear plaque; little or no growth on JL5434JACGCA c $,.YesNo/,  , : AM PMwd  A#F#CH ^#NB  A$A )95  0):9h CTAphenotypes and diffsBz @,A  $,.YesNo/,  , : AM PMB   M $,.YesNo/,  , : AM PMN   O $,.YesNo/,  , : AM PMP   Y $,.YesNo/,  , : AM PMwZ  [ $,.YesNo/,  , : AM PMw\  1P: inIW`WcWeWrWxWMWinlysogenyteststreakisthinandwatery-lookingcoloniesIVisIWjl5434GWHWIWJWKWLWMWNWOWlargeHWKWlargerHWless-turbidKWlittleGWHWIWJWKWLWMWNWOWlysogenyHWIWKWlysogenytestseesomevvtinycolonieslargernextdayplaHVHVHVgernextdayplaHVVayplaHVVrnextdayplaHVVdayplaHVVlaHVVplaHVVvtinycolonieslargernextdayplaHVVHVVVVO$ morerWmutationOWcWeWMWmutationnearxhoisiteincie10veVmutationscWxWneareWnextHWKWnocWeWxWGWHWIWJWKWLWMWNWOWnomutationinciicVeVnomutationsinciixVnothingKWonGWHWIWJWKWLWMWNWOWoneKWorKWGWHWIWJWKWLWMWNWOWor1OWor2OWcWor3cWphenotypeKWplaque`WjWrWGWHWIWJWKWLWMWNWOWplaquesHWJWKWLWMW`WjWrWplaquesareuniformLVplaquesareuniforminturbidityacrossplaque`VplaquesareuniformlyturbidacrossplaquejVplaqueshaveclearishcentralzoneJVplaqueshavelargeless-turbidcentralzonelysogenytestseKVplaqueshavesmallclearishcentralzoneMVplaquesmoreuniforminturbidityacrossplaquerVplateQWnWplatestock4/6/01QVnVplatesKWpositionOWMWposncWroomKWsatKWseeHWKWsideOWcWsiteeWslow-growthKWsmallIWKWMWsomeHWKWspacerUWspacerforcolumnsUVstockQWnWnWnWnWnWWnWWW$m aaatGacaa3acac$acct'acgc"LactaHMactc?agcc atacKcaaa:caac caccJcacgIcact8cagc_ (9!0");# #$B strain nameNplaque phenotypeUS Plysogenization?ZpRM -35\-10^totaldiffs from WT_WT=GATA`GATTbdiffs fromconsd AT bp in -10 @B9A B9%A B^         TU A<Wa #^;mZ  $A<V *AP5  %0A:Ph D$TBAqPc4%3:%>3_ @O!02"AS# 5$UVw) \A Layout #1B$$$$@A  $,.YesNo/,  , : AM PMB   C $,.YesNo/,  , : AM PMD   E $,.YesNo/,  , : AM PMF   G $,.YesNo/,  , : AM PMH   I $,.YesNo/,  , : AM PMc  AcolumnsB@$$@ A  B  E  $,.YesNo/,  , : AM PMwG  $,.YesNo/,  , : AM PMwH  $,.YesNo/,  , : AM PMwL  $,.YesNo/,  , : AM PMwM  $,.YesNo/,  , : AM PMwM     #TTU2V2W4]3^1_4`2"A2CAACACGCACGGTGTTAGACCTTTATCCCTTGCGGTGATAGTGTTAACGT2@A>  *  0  >d1.5      >hnoiturbidjmediumk2 ++ %AMD36B36CMDGyesHv faintly turbid in center; some are larger and clear (may be clears). After two days, more turbid in center. Looks like a cII mutant might look.I6Mutation near XhoI site in cI, E10V No mutation in cIIJCCACKAAGTMCNCOAPAQCRASGTTU0V2W6]0^2_6`3"A2CAACACGCACGGTGTTACCACTTTATCCCTTGCGGTGATAAAGTTAACGT2@A>  *  0  >d1.5caacacgcacggtgttagac#caacacgcacggtgttagatcaacacgcacggtgttagca,caacacgcacggtgttagcccaacacgcacggtgttagct2caacacgcacggtgttaggacaacacgcacggtgttaggc&.caacacgcacggtgttagggcaacacgcacggtgttatac45caacacgcacggtgttatag!caacacgcacggtgttatca caacacgcacggtgttatcc-1caacacgcacggtgttatct =caacacgcacggtgttatgc/caacacgcacggtgttattc777HP LaserJet IIId A4db1CAACACGCACGGTGTAGCACTTTATCCCTTGCGGTGATAAACTTAACGThe  B@"\2s@"2!@"2#@"2%@*?'("2)@*"2<+@*,C"G3c-@AX."n3/j0"312"334xe=  .A total diffs @AH J?4Hplike cons: -35r-104Adiffs from consensus@AH ?H @BABA" "B6                  u 6.144207e-06@6.144207e-06@7.465853e-04@3e-04@7.465853e-04@7.465853e-04@7.465853e-04@7.465853e-04@8.395999e-03@999e-03@8.395999e-03@8.395999e-03@8.395999e-03@8.395999e-03@7.889319e-06@7.889319e-06@7.889319e-06@9319e-06@9319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@7.889319e-06@8.231145e-05@1145e-05@-04@05@+ AJ @XO )@MOcMBA@S(3@ V6a7m;A@CI@85MDUEG@C@N]TAW@UPVSq @aBKhj@Fk [peoqhB]@e.A^= ]iA%@dPcY i H* I>N9bKnh O#TZ%j )b-"A1P5Ul8H;??"A2IHLLK?mdcGHIJKLMNOU1`W3/24`W3/24nobut1colony`VbutWW\W`WcWfWgWxWcolony`WfewWW\WcWfWgWxWWWWWdY  Plysogenization?ZpRM -35\-10^totaldiffs from WT_WT=GATA`GATTbdiffs fromconsd AT bp in -10 @B!A B!%A BF      %c' B strain nameNplaque phenotype#CH ^#NB  A$A )95  0):9h BDT)q9f:>_ (9!0");# #$B strain nameNplaque phenotype]^P B strain nameT pRM min35V pRM min10g-35h-10jlike WT: -35 l-10.!@"2#@"2%@*?'(!1)@*!15+@*,C @1\-@AX.#j4/j0#412#434xB strain nameT pRM min35V pRM min10g-35h-10jlike WT: -35 l-10.\.u"#$%(fewWW\WcWfWgWxWno $)*?ntGHIJKLMNOunstable 0139yes WW\W!"cW%fWgW(+,-./24567xW:;<=>yesfewWV\VcVfVgVxV)*+AAz(HHR[ (hh t2 HK'd!5So zAJHP Color LaserJet 4600 PS odXX0]Se֡d4d E7@@QBUUX   $,.YesNo/,  , : AM PM@ HelveticaGenevaArial Courier NewSymbolTimes New RomanWebdings MS Sans Serif AvantGarde !@A! xVDEF(LIST.LISTA.>WT >=WT ~WT <=WT 0.5  *  0  >chtwo types when coplated; probably 52 has larger clear center than WT, but cant be sure which is lighterd <<WTe5hyesiturbidjlargek4 ++++7AMD54B54CMDGyesHlarge, fairly turbidJTTCGKCGATMTNTOCPCQGRGSATTU0V1W7]1^2_5`2b2CAACACGCACGGTGTTATTCGTTTATCCCTTGCGGTGATACGATTAACGTd <<gacc#gacggactgatagcacgcat,gccagcctgcttggacggca...a..KTGAAMGNAOCPTQGRGSATAU2V0W6]3^2_3`3b2CAACACGCACGGTGTTAGACGTTTATCCCTTGCGGTGATATGAATAACGTd=WTe4.5hyesiturbidjlargek4 ++++AMD26B26CMDGyesHlarge, very turbid, poss >WTJGCCTKGGCTMGNCOCPGQTRGSCTTU1V2W5]2^1_5`1b2CAACACGCACGGTGTTAGCCTTTTATCCCTTGCGGTGATAGGCTTAACGTcturbid, about like WTd~WTe4hyesiturbidjlargek 4.5 ++++(+)AMD27B27CMDGyes, rel few but healthyHsmall->medium, pretty turbidJGGACKTAGAMGNGOAPTQCRASGTAU1V1W6]1^2_5`3b2CAACACGCACGGTGTTAGGACTTTATCCCTTGCGGTGATATAGATAACGTd~WTe4AACGTph  e $,.YesNo/,  , : AM PMwg  h  i $,.YesNo/,  , : AM PMwj  k $,.YesNo/,  , : AM PMwl  m $,.YesNo/,  , : AM PMwn , o $,.YesNo/,  , : AM PMwp  ko P [ $,.YesNo/,  , : AM PMw] $,.YesNo/,  , : AM PMw_ $,.YesNo/,  , : AM PMwa $,.YesNo/,  , : AM PMwc $,.YesNo/,  , : AM PMwu`oEX STRAIN NAME'@4 B&A+ AJ @XO )@MOcMBA@S(3@ V6a7m;A@CI@85MDUEG@C@N]TAW@UPVSVS 0) wwbTwwbTT/ +UB0CMDCI(spacer for columns)h:md58;i;md59<jr>md62?s?mdc6Lp@UR DG_qmd1@vmdc2@w@bhealthy@yesZclearish@,largefaintlyturbid@!largeprettyturbidthereisalargeless-turbidbutnotcle@largeturbidveryslightclearishdotincenter@or@-sl@ a@1in@Omore@Pstreak@$Q ccacCmggct@"tcag@: gtct@B0g"caacacgcacggtgttaaaa@[caacacgcacggtgttaaca@\fB.@#RtEGIKMOQSUWY[]_acegikmoqsuwy{}y{}y{}y{}y{}y{}{}y{}cMBA@S(3@ V6a7m;A@CI@85MDUEG@C@N]TAW@UPVSwmd1md10 md11 md12 md13md14md15md16md17md18md19md2md20md21md22md23md24md25md26md27md28md29md3md30md31 md32!md33"md34#md35$md36%md37&md38'md39(md4md40)md41*md42+md43,md44-md45.md46/md470md481md492md5md503md514md525md536md547md558md569md57:md58;md59md62?md7md8 md9 mdc1Gmdc10Pc1G1Gc1G1Gc1G1Gc1G1Gc1G1Gc1G1Gc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc1G1Gmdc10PWvbmdc2Hmdc3Imdc4Jmdc5Kmdc6Lmdc7Mmdc8Nmdc9OU G0H@IPJ`KpLM N O‚! ‚" ‚# ‚$‚%‚&‚'‚(‚)‚*‚1‚2‚3‚4‚5‚6‚7‚8‚9‚:‚A‚B ‚C!‚D"‚E#‚F$‚G%‚H&‚I'‚J(‚Q)‚R*‚S+‚T,‚U-‚V.‚W/‚X0‚Y1‚Z2‚a3‚b4‚c5‚d6‚e7‚f8‚g9‚h:‚i;‚j<‚q=‚r>‚s?i;‚j<‚q=‚r>‚s??