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Highlighted titles link to the abstract, highlighted journal names link directly to the PubMed citation. PubMed contains abstracts, links to related articles, DNA and protein sequences, if available, and other useful information.  Note: * denotes an undergraduate contributor.

Elizabeth Vierling CV
 

New Publications 

Schramm, F., J. Larkindale, K. Kiehlmann, G. Arnab, G. Englich, G., E. Vierling, P. von Koskull-Döring. A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis. Plant J. 53: 264-274 (2008).

Painter, A.J., N. Jaya, E. Basha, E. Vierling, C.V. Robinson , J.L. Benesch. Real-Time Monitoring of Protein Complexes Reveals their Quaternary Organization and Dynamics. Chem Biol. 15:246-53. (2008)

McClellan, C.A., T.J. Turbeyville, E.M. K. Wijeratne, A. Kerschen, E. Vierling , C. Queitsch, L. Whitesell, A.A. Gunatilaka. A rhizosphere fungus enhances Arabidopsis thermotolerance through production of an Hsp90 inhibitor. Plant Physiol. (2007).

Kotak, S., E. Vierling , H. Bäumlein, P. von Koskull-Döring. A novel transcriptional cascade regulating heat stress proteins during seed development in Arabidopsis. Plant Cell 19:182-195 (2007).

Kwon, Y., Kim, S-H., Jung, M-S., Kim, M-S., Oh, J-E., Ju, H-W., Kim, K-I., Vierling, E ., Lee, H., Hong, S-W. Arabidopsis hot2 encodes an endochitinase-like protein that is essential for tolerance to heat, salt and drought stresses. Plant J. 49:184-193 (2007).

Lee, U., I. Rioflorido, S-W. Hong, J. Larkindale, E. R.Waters, E.Vierling . The Arabidopsis ClpB/Hsp100 family of proteins: Chaperones for stress and chloroplast development. Plant Journal 49:115-127 (2007).

Reviews

Siddique, M., S. Gernhard, P. von Koskull-Döring, E. Vierling, K-D. Scharf. The plant sHSP superfamily: Five new members in Arabidopsis thaliana with unexpected properties. Cell Stress & Chaperones 13:183-197  (2008).

Larkindale, J., E. Vierling. Core genome responses involved in acclimation to high temperature. Plant Physiol. 146: 748-761 (2008).

Vierling, E. Mechanism of chaperone action of small heat shock proteins, in ed. W. Houry, Molecular Chaperones: Principles and Diseases (Henry Stewart Talks, London , 2007). Series info webpage: http://www.hstalks.com/molchap/index.htm

Kotak, S., J. Larkindale, U. Lee, P. von Koskull-Döring, E. Vierling , K-D. Scharf. Complexity of the heat stress response in plants. Curr. Opin. Plant Biol., 10:310-316 (2007).

Larkindale, J., Mishkind, M., E. Vierling . Plant responses to high temperature: In: Plant Abiotic Stress . Matthew A. Jenks and P.M. Hasegawa, eds. Blackwell Publishing. (2005).

van Montfort, R., C. Slingsby, E. Vierling . Structure and function of the small heat shock protein/a-crystallin family of molecular chaperones. In: Protein Folding in the Cell . Advances in Protein Chemistry Series. A. Horwich, ed. Academic Press. Vol 59:105-156 (2002).

Scharf, K-D., M. Siddique, E. Vierling .The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing α-crystallin domains (Acd proteins). Cell Stress & Chaperones 6:225-237 (2001).

Vierling, E. (1997). Plant HSP101/ClpB. In: Guidebook to molecular chaperones and Protein-Folding Catalysts. M.J. Gething, ed. Sambrook and Tooze Publications at Oxford University Press. pp.253-255 

Vierling, E. (1997). Chloroplast-localized Clp proteins. In: Guidebook to molecular chaperones and Protein-Folding Catalysts. M.J. Gething, ed. Sambrook and Tooze Publications at Oxford University Press. pp.255- 258. 

Gaestel, M., E. Vierling, Buchner, J. (1997). The small heat shock protein (sHSP) family - an overview. In: Guidebook to molecular chaperones and Protein-Folding Catalysts. M.J. Gething, ed. Sambrook and Tooze Publications at Oxford University Press. pp.269-272. 

Vierling, E., Lee, G.J. (1997). Plant small heat shock proteins (sHSPs). In: Guidebook to molecular chaperones and Protein-Folding Catalysts. M.J. Gething, ed. Sambrook and Tooze Publications at Oxford University Press. pp.277-280. 

E. Vierling. (1997). The small heat shock protein in plants are members of an ancient family of heat induced proteins. Acta Physiologiae Plantarum, 19:539-547. 

Boston, R.S., P.V. Viitanen, E. Vierling. (1996). Molecular chaperones and protein folding in plants. In: Post-transcriptional control of gene expression in plants, W. Filipowicz and T. Hohn. Plant Mol. Biol., 32:191-222. 

Waters, E.R., Lee, G.J. and Vierling, E. (1996). Evolution, structure and function of the small heat shock proteins in plants. J. Exp. Bot. 47: 325-338. 

Vierling, E. (1991). The roles of heat shock proteins in plants. Annu. Rev. Plant Physiology Plant Mol. Biol. 42: 579-620. 

Small HSPs, chloroplastic

Waters, E., E. Vierling. (1999). Chloroplast small heat shock proteins: Evidence for atypical evolution of an organelle-localized protein. Proc. National Acad. Sciences. 96:14394-14399.

Harndahl,U., R.B. Hall, K.O. Osteryoung, E. Vierling, J. Bornman, C. Sundby. (1999). The chloroplast small heat shock protein undergoes oxidation-dependent conformational changes and may protect plants from oxidative stress. Cell Stress & Chaperones. 4:129-138.

Suzuki, T.C., D.C. Krawitz, E. Vierling. (1998). The chloroplast small heat shock protein oligomer is not phosphorylated and does not dissociate during heat stress in vivo. Plant Physiol. 116:1151-1161. 

Osteryoung, K.W. and Vierling, E. (1994). Dynamics of small heat shock protein distribution within the chloroplasts of higher plants. J. Biol. Chem. 269, 28676-28682. 

Chen, Q., K. Osteryoung and E. Vierling. (1994). A 21-kDa chloroplast heat shock protein assembles into high molecular weight complexes in vivo and in organelle. J. Biol. Chem. 269:13216-13223. 

Chen, Q. and Vierling, E. (1991). Analysis of conserved domains identifies a unique structural feature of a chloroplast heat shock protein. Mol. Gen. Genet. 226, 425-431. 

Small HSPs, cytosolic

Helm, K.W., Lee, G.J., and Vierling, E. (1997). Expression and Native Structure of Cytosolic Class II Small Heat Shock Proteins. Plant Physiol. 114(4): 1477-1485. 

Hernandez, L.D. and Vierling, E. (1993). Expression of low molecular weight heat-shock proteins under field conditions. Plant Physiol. 101: 1209-1216. 

DeRocher, A.E., Helm, K.W., Lauzon, L.M. and Vierling, E. (1991). Expression of a conserved family of cytoplasmic low molecular weight heat shock proteins during heat stress and recovery. Plant Physiol. 96,1038-1047. 

Small HSPs, endomembrane-localized 

Helm, K.W., P. Lafayette, R.T. Nagao, J.L. Key and E. Vierling. (1993). Localization of small HSPs to the higher plant endomembrane system. Mol. Cell. Biol. 13:238-247. 

Small HSPs, expression during seed development 

Wehmeyer, N., E. Vierling. The expression of sHsps in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiol., 122: 189-198 (2000).

DeRocher, A.E. and E. Vierling. (1994). Developmental control of small heat shock protein expression during pea seed maturation. Plant J. 5:93-102. 

Wehmeyer, N., L.D. Hernandez, R.R. Finkelstein, E. Vierling. (1996). Synthesis of small heat shock proteins is part of the developmental program of late seed maturation. Plant Physiol. 112:747- 757. 

Small HSPs, molecular chaperone activity

Jaya, N., V. Garcia*, E. Vierling. Substrate binding site flexibility of the small heat shock protein molecular chaperones. Proc. Natl. Acad. Sci. (2009), In press.

Cheng, G., E. Basha, V.H. Wysocki, E. Vierling. Insights into small heat shock protein and substrate structure during chaperone action derived from hydrogen/deuterium exchnage and mass spectrometry. J. Biol. Chem., 283:26634-42 (2008) Featured as “Paper of the Week”. PMID: 18621732

Basha, E., K.L. Friedrich, E. Vierling . The N-terminal arm of small heat shock proteins is important for both chaperone activity and substrate specificity. J. Biol. Chem. 281: 39943-39952 (2006).

Giese, K.C., E. Basha, B.Y. Catague, E. Vierling . Evidence for an essential function of the N-terminus of a small heat shock protein in vivo, independent of in vitro chaperone activity. Proc. Natl. Acad. Sci. 102: 18896-18901(2005).

Giese, K.C., E. Vierling . Mutants in a small heat shock proteins that affect the oligomeric state: analysis and allele specific suppression. J. Biol. Chem. 279: 32674 - 32683 (2004).

Basha, E., G.J. Lee, B. Demeler, E. Vierling . Chaperone activity of cytosolic small heat shock proteins in wheat. Eur. J. Biochem. 271:1-11 (2004).

Basha, E., G. J. Lee, L. A. Breci, A.C. Hausrath, N. R. Buan, K C. Giese, E. Vierling .The Identity of Proteins Associated with a Small Heat Shock Protein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions. J. Biol. Chem., 279: 7566-7575 (2004).

Friedrich, K. L., K. C. Giese, N. R. Buan, E. Vierling . Interactions between small heat shock protein subunits and substrate in small heat shock protein/substrate complexes. J. Biol. Chem. 279:1080-1089 (2004).

Mogk, A., E.Deuerling, S. Vorderwülbecke, E. Vierling , B. Bukau. Small heat shock proteins, ClpB and the DnaK system form a functional triade in reversing protein aggregation. Mol. Microbiol. 50:585-595. (2003).

Wintrode, P.L., K. L. Friedrich, E. Vierling , J. B. Smith, D. L. Smith. Solution structure and dynamics of a heat shock protein complex probed by hydrogen exchange/mass spectrometry. Biochemistry 42:10667-10673 (2003).

Mogk, A., C. Schlieker, K. L. Friedrich, H-J. Schönfeld, E. Vierling , B. Bukau. Refolding of substrates bound to small Hsps relies on a disaggregation reaction mediated most efficiently by ClpB/DnaK J. Biol. Chem. 278:31033-31042 (2003).

Giese, K.C., E. Vierling . Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J. Biol. Chem. 277: 46310-46318 (2002)

Sobott, F., J.L.P. Benesch, E. Vierling , C.V. Robinson. Subunit exchange of multimeric protein complexes Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray-mass spectrometry. J. Biol. Chem. 277: 38921-38929 (2002).

van Montfort, R., E. Basha, K.L. Friedrich, C. Slingsby, E. Vierling . Structure and assembly of a eukaryotic small heat shock protein. Nature Struct. Biol. 8:1025-1030 (2001).

Lee, G.J., E. Vierling. (2000). A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein. Plant Physiology. 1:189-198.

Lee, G.J. and Vierling, E. (1998). Expression, Purification and Molecular Chaperone Activity of Plant Recombinant Small Heat Shock Proteins. In: Protein Folding:catalysts, accessory proteins, and chaperones. Eds. G. Lorimer and T.O. Baldwin. Methods in Enzymology. 29:350-365. 

Lee, G.J., A.M. Roseman, H.R. Saibil, E. Vierling. (1997). A small heat shock protein stably binds heat- denatured model substrates and can maintain a substrate in a folding competent state. EMBO J. 16:659-671. 

Lee, G.J. (1995). Assaying proteins for molecular chaperone activity. Methods Cell Biol. 50, 325-333. 

Lee, G.J., Pokala, N., and Vierling, E. (1995). Structure and in vitro molecular chaperone activity of cytosolic small heat shock proteins from pea. J. Biol. Chem. 270:10432-10438.

Small HSPs, molecular evolution 

Basha, E.M., E.R. Waters, E. Vierling. (1999).Triticum aestivum cDNAs homologous to nuclear-encoded mitochondrion-localized small heat shock proteins. Plant Science. 141:93-103. 

Waters, E.R, E. Vierling. (1999). The diversification of plant cytosolic small heat shock proteins preceded the divergence of mosses. Molecular Biol. & Evolution. 16:127-139. 

Thermotolerance 

Lee, U., C.Wie*, B. O. Fernandez , M. Feelisch , E. Vierling. Modulation of nitr osative stress by S-nitrosoglutathione reductase is critical for thermotolerance and plant growth. Plant Cell 20: 786-802, (2008).

Bologi, Z., O. Cheregi, K.C. Giese, K. Juhász, E. Vierling, I. Vass, L. Vigh, I. Horváth. A mutant small heat shock protein with increased thylakoid association provides an elevated resistance against UV-B damage in Synechocystis 6803. J. Biol. Chem. 283:22983-22991 (2008).

McClellan, C.A., T.J. Turbeyville, E.M. K. Wijeratne, A. Kerschen, E. Vierling, C. Queitsch, L. Whitesell, A.A. Gunatilaka. A rhizosphere fungus enhances Arabidopsis thermotolerance through production of an Hsp90 inhibitor. Plant Physiol 145: 174-182 (2007).

Larkindale, J. J, D. Hall, M. R. Knight, E. Vierling . Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol., 138:882-97 (2005).

Clerkx,E.J.M., M. E. El-Lithy, E. Vierling , G.J. Ruys, H.Blankestijn-DeVries, S.P.C. Groot, D. Vreugdenhil, M. Koornneef. Analysis of natural allelic variation of Arabidopsis seed quality traits between the accessions Landsberg erecta and Shakdara, using a new recombinant inbred line population. Plant Physiol. 135: 432-443 (2004).

Liu, Z., S-W. Hong, M. Escobar, E. Vierling , D. L. Mitchell, D. W. Mount, J. D. Hall. Arabidopsis UVH6, a homolog of human XPD and yeast RAD3 DNA repair genes, functions in DNA repair and is essential for plant growth. Plant Physiol. 132:757-767 (2003).

Hong, S-W., U. Lee, E. Vierling .Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures. Plant Physiol. 132:1405-1414 (2003).

Salvucci, M. E., K.O. Osteryoung, S.-J. Crafts-Brandner, E. Vierling . Exceptional sensitivity of rubisco sctivase to thermal denaturation in vitro and in vivo. Plant Physiol. 127:1053-1064 (2001).

Small HSPs, and lipids

Balogi,Z., Z. Török, G. Balogh, K. Jósvay, N. Shigapova, E. Vierling , L. Vígh, I Horváth.Heat shock lipid in cyanobacteria during heat/light-acclimation. Arch. Biochem. Biophys. Membrane Biochem. Biophys. 436:346-54 (2005).

Tsvetkova, N.M., I. Horváth, Z. Török, W.F. Wolkers,, Z. Balogi, N. Shigapova, L.M. Crowe, F. Tablin, E. Vierling , J.H. Crowe, L. Vigh. Small heat shock proteins regulate lipid polymorphism. Proc. Natl. Acad. Sci. 99:13504-13509 (2002).

Török, Z., P. Goloubinoff, I. Horváth, N.M. Tsvetkova, A. Glatz, G. Balogh, V. Varvasovszki, D.A.Los, E. Vierling , J.H. Crowe and L. Vígh. HSP17 is an amphitropic protein that stabilizes heat-stressed membranes and binds denatured proteins for subsequent chaperone-mediated refolding. Proc. Natl. Acad. Sci. 98:3098-3103 (2001).

Hsp70 

Sung, D.Y., E.Vierling , C. Guy. Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family. Plant Physiol. 126:789-800 (2001).

DeRocher, A., and Vierling, E. (1995). Cytoplasmic HSP70 homologues of pea: differential expression in vegetative and embryonic organs. Plant Mol. Biol. 27:441-456. 

Hsp100

Tonsor, S.J., C. Scott, I. Boumanza*, T.R. Liss,  J.L. Brodsky, E. Vierling. Heat shock protein 101 effects in Arabidopsis thaliana: Genetic variation, fitness and pleiotropy in controlled environments. Mol. Ecol., 17: 1614-1626 (2008).

Lee,U., C. Wie, M. Escobar, B. Williams, S.-W. Hong, E. Vierling . Genetic analysis reveals domain interactions of Arabidopsis Hsp100/ClpB and cooperation with the sHsp chaperone system. Plant Cell, 17:559-571 (2005).

Lum, R., J. M. Tkach, E. Vierling , and J. R. Glover.Evidence for an Unfolding/Threading Mechanism for Protein Disaggregation by Saccharomyces cerevisiae Hsp104. J. Biol. Chem. 279: 29139 - 29146 (2004).

Hong, S-W., E.Vierling . Hsp101 is necessary for heat tolerance but dispensable for development and germination in the absence of stress. Plant Jour. 27:25-35 (2001).

Hong, S-H., E. Vierling.Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. Proc. Natl. Acad. Science, 97: 4392-4397 ( 2000).

Queitsch,C., S-H. Hong, E. Vierling, S. Lindquist. Heat Shock Protein 101 Plays a Crucial Role in Thermotolerance in Arabidopsis.Plant Cell, 12: 479-492 (2000).

Schirmer, E.C., Lindquist, S., and Vierling, E. (1994).An Arabidopsis heat shock protein complements a thermotolerance defect in yeast. Plant Cell. 6:1899-1909.